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Updated: Jun 26, 2026

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
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在血中丰富微生物DNA以改善败血症病原体检测:试点研究

Eddie G Dominguez1,2, Bradon R McDonald1,2, Haikun Zhang1,2

  • 1Department of Surgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

Clinical chemistry
|March 11, 2025
PubMed
概括

结合单链DNA (ssDNA) 库的准备和大小选择,可以提高微生物DNA在血中的产量. 然而,这种方法增加了背景噪声,限制了在属级的病原体检测灵敏度.

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MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...