在实验室中,使用静态INFOGEST消化模型和肠道透性模型,评估从化乳酸细菌中获得的生物可用性
Iva Mrvikova1, Ivana Hyrslova1, Vera Nesporova2
1Dairy Research Institute Ltd., Prague, Czech Republic; Department of Microbiology, Nutrition, and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Czech Republic.
概括
乳酸细菌产生的纳米粒子 (SeNPs) 具有很高的生物可访问性,用于食补充剂. 这些SeNPs为增强吸收和潜在的益生菌益处提供了化酵母的有希望的替代品.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 营养科学 营养科学
背景情况:
- 微生物 (Se) 补充研究非常重要,因为不同形式的的生物可用性和毒性各不相同.
- 化酵母是常见的,但乳酸细菌 (LAB) 提供了独特的特性和益生菌的潜力,用于Se.交付.
- LAB可以增强肠道微生物群和胃肠道健康,为Se补充添加价值.
研究的目的:
- 研究来自两个特定的乳酸细菌菌株的的体外生物可访问性和生物可用性.
- 在模拟消化过程中评估Se积累,物种化和稳定性.
- 通过肠道细胞模型来评估Se透.
主要方法:
- 两个LAB菌株 (Streptococcus thermophilus CCDM 144和Enterococcus faecium CCDM 922 A) 用于Se的积累.
- 在模拟胃肠道消化过程中分析了的物种化和稳定性.
- 使用Caco-2细胞单层模型研究了透性.
主要成果:
- LAB 主要将Se代谢成纳米颗粒 (SeNP,64-77%).
- 有机Se物种在消化过程中显示出高的生物可用性 (高达90%),但低的生物可用性 (<0.1%).
- SeNPs表现出高生物可访问性 (~90%) 和适度的肠道输送 (16-19%在4小时内).
结论:
- 由LAB生产的纳米颗粒是一种高度生物可访问的Se形式,用于食补充剂.
- 需要进一步的研究来了解SeNP在人体中的行为,以便安全有效的营养应用.
相关概念视频
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