从乙驱动的部分脱 (PD) 到N-甲基利驱动的PD:微生物群体,代谢途径和功能基因
Longxiao Bai1, Shaopo Wang1, Lingjie Liu1
1School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26, Tianjin 300384, China; Tianjin Key Laboratory of Aquatic Science and Technology, Jinjing Road 26, Tianjin, China.
Journal of hazardous materials
|March 11, 2025
概括
N-Methylpyrrolidone (NMP) 可以作为一种低成本的碳来源,用于在废水处理中驱动脱. 这项研究使细菌适应使用NMP,实现了高去除和积累酸盐.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 电池生产产生了大量含有N-甲基 Pyrrolidone (NMP) 的废水.
- 为含有NMP的废水开发具有成本效益的生物处理方法至关重要.
- NMP是一种极性近极溶剂,广泛用于电池制造.
研究的目的:
- 调查使用NMP作为部分脱 (PD) 过程中的碳来源的可行性.
- 为了使微生物群落适应使用NMP在测序批量反应堆 (SBR) 中去除.
- 在TOC去除和酸盐转化为酸盐方面评估NMP驱动PD的效率.
主要方法:
- 在SBR中通过逐渐用NMP取代酸来适应PD系统.
- 监测酸盐转化为酸盐的转化率 (NTR),TOC去除和NO3-N减少.
- 使用分子技术分析微生物社区结构和NMP代谢相关基因的丰富性.
主要成果:
- 经过适应的PD污泥显示稳定的NTR约为60%,高达68.1%.
- TOC的去除超过了90%,NO3-N的减少达到96.7%.
- 脱细菌Paracoccus和NMP代谢基因 (hyuA,hyuB,mao,gabD) 的相对丰度显著增加,这表明Paracoccus发挥了关键作用.
结论:
- NMP可以有效地作为碳源来驱动PD过程,从而导致酸盐的积累.
- 本研究提出了一种使用NMP从电池废水中去除的新策略.
- 这些发现突出了帕拉科库斯在NMP生物降解和去除方面的潜力.
更多相关视频
06:17Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
27.5K
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
9.2K
相关概念视频
01:20Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K
01:20Overview of Nitrogen Metabolism
7.8K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.8K
00:46Bioremediation
18.1K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.1K
01:29Fermentation
113.0K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
113.0K