Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.3K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.5K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.5K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.3K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.1K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Phase Engineering of Iridium Oxides Enables Direct Coupling of Proton Exchange Membrane Water Electrolysis With Intermittent Electrical Energy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Integrating Solution Physical Properties into Zeolite Synthesis Prediction via Causal Machine Learning.

The journal of physical chemistry letters·2026
Same author

Efficient and Safe Membrane-Free Flow Electrolyzer for Formate Synthesis and Direct Fuel Cell Integration.

Angewandte Chemie (International ed. in English)·2026
Same author

AI-Assisted Electron Microscopy in Structure-Performance Analysis of Advanced Catalysts: From Atomic Resolution to Statistical Significance.

Nano letters·2026
Same author

Ginsenoside Rb2 modulates the skin barrier by targeting Src to regulate PI3K/Akt signaling in HaCaT cells.

Journal of ginseng research·2026
Same author

Sulfur-passivated Pt cluster edges on CeO<sub>2</sub> for selective CO<sub>2</sub>-to-CO conversion.

Nature communications·2026

相关实验视频

Updated: May 23, 2025

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.1K

在电子显微镜中通过增强的限制效应对分子结构和相互作用进行成像.

Mengmeng Ma1,2, Qinnan Yu1,2, Jiayi Zhang1,2

  • 1Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou, 215123, Jiangsu, China.

Nature communications
|March 12, 2025
PubMed
概括

稳定的分子原子成像是通过限制和低剂量电子显微镜实现的. 更强大的宿主-客人相互作用提高了分子图像的清晰度,并使分子行为研究成为可能.

更多相关视频

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.8K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K

相关实验视频

Last Updated: May 23, 2025

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.1K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.8K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K

科学领域:

  • 材料科学 材料科学 材料科学
  • 化学 化学 化学
  • 物理 物理学 物理

背景情况:

  • 对分子及其相互作用的原子成像对于理解基础物理和化学至关重要.
  • 电子显微镜的挑战包括分子热流动性和光束灵敏度.

研究的目的:

  • 提出使用低剂量电子显微镜对受限分子进行成像的一般策略.
  • 评估各种材料系统中宿主-客人相互作用的强度.
  • 分析相互作用强度对分子成像质量的影响.

主要方法:

  • 在电子显微镜中利用封闭效应和低剂量成像技术.
  • 研究三种材料系统:岩石,岩石和金属有机框架.
  • 分析分子投影使用面积比与相互作用强度相关联.

主要成果:

  • 在矿 (离子) 和岩 (范德瓦尔斯) 系统中,通过增强宿主-客人相互作用,获得了分子配置的清晰图像.
  • 在金属有机框架 (协调) 系统中解决了芳香物的原子结构和键.
  • 建立了分子图像质量和宿主-客人相互作用强度之间的关系.

结论:

  • 低剂量电子显微镜与限制为高分辨率分子成像提供了可行的策略.
  • 主机-客户互动强度显著影响原子尺度分子图像的清晰度和细节.
  • 这种方法使得在各种应用中能够在现实空间中研究各种分子行为.