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生产,隔离,优化和表征来自澳大利亚白菌的微生物PHA

Rafwana Ibrahim¹, Jesil Mathew Aranjani², Navya Prasanna¹

¹Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, 576140, India.

Scientific reports

|March 12, 2025

概括

研究人员对土壤微生物进行了选,以寻找生物塑料替代品聚酸酸盐 (PHAs). 最好的菌株Bacillus australimaris (GS-14),表现出高的PHA产量,并以其特性为特征.

科学领域:

微生物学微生物学
生物技术是生物技术.
聚合物科学聚合物科学

背景情况:

由于不可降解物质造成的环境污染日益增加,需要可持续的替代品.
微生物聚合物的聚烯酸酸盐 (PHAs) 为生物塑料生产提供了可生物降解的解决方案.
在营养有限的条件下,微生物会积累PHAs.

研究的目的:

用于选土壤微生物的多基酸盐 (PHA) 生产.
为了优化介质参数以提高PHA产量.
为了描述产生的PHA,并确定生产的生物体.

主要方法:

使用苏丹黑色染色分离和选土壤微生物.
通过二酸盐方法识别有前途的GS-14菌株.
使用克罗酸标准曲线量化PHA.
使用FTIR,LC-MS和DSC进行聚合物表征.
通过16S rRNA测序进行细菌鉴定.
使用 Minitab 软件和统计分析进行媒体优化.

主要成果:

17个土壤隔离物被证实用于PHA生产.
株GS-14表现出最高的PHA产量.
FTIR证实了PHA特征的峰值 (C=O延伸在1732厘米-1).
LC-MS表示一种寡合物种 (641.6 g/mol);聚合物MW估计为5,00020,000 Da.
DSC显示了174°C的外热峰值,使得结晶度计算成为可能.
澳大利亚菌被确定为产生PHA的有机体 (GS-14).

结论:

澳大利亚菌株GS-14是工业PHA生产的有希望的候选人.
优化媒体参数可以提高PHA产量.
鉴定证实了生物聚合物的特性,适合生物塑料.
这项研究有助于从微生物来源开发可持续的生物塑料.

关键词:

它是生物降解的.生物塑料的生物塑料生物聚合物的生物聚合物.微生物聚合物的聚合物.聚酸酸多基甲酸隔离土壤隔离土壤

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