Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

175
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
175
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

6.6K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Multipart Phase 1 Study to Assess Safety and Pharmacokinetics of Ibuzatrelvir in Healthy Participants and Dose Selection for a Dose-Ranging Study.

Clinical and translational science·2026
Same authorSame journal

Updated Recommendations for the Bioanalysis of Antibody-Drug Conjugates (ADC) from the ADC working group of the AAPS Bioanalytical Community.

The AAPS journal·2026
Same author

Complete Genomes of Human Papillomavirus Type 16 Viruses Isolated from Cases of Cervical Neoplasia and Squamous Cell Carcinomas Followed in Latvia in 2012-2024.

Vaccines·2026
Same author

Thorough QT/QTc Clinical Study to Evaluate the Effect of Remdesivir on Cardiac Repolarization in Healthy Participants.

Clinical and translational science·2026
Same author

Predicting human pharmacokinetic parameters of drugs using a multi-tissue chip platform integrating liver, kidney, and skeletal muscle microphysiological systems.

Lab on a chip·2026
Same author

Advancing IBD Management: A Literature Review on the Role of Non-Invasive Blood-Based Biomarkers in Predicting and Assessing Pharmacodynamic Response to Treatment.

Clinical and translational science·2026

相关实验视频

Updated: May 23, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.1K

非液态矩阵生物分析的最佳实践和建议

Faye Vazvaei-Smith1, Wenkui Li2, Omar S Barnaby3

  • 1Merck & Co., Inc., West Point, Pennsylvania, 19486, USA. fataneh.vazvaei-smith@merck.com.

The AAPS journal
|March 12, 2025
PubMed
概括

本文提供了在药物开发中分析非液态矩阵 (NLM) 的基本最佳实践. 它解决了挑战,并为定量NLM生物分析提供了指导,以推进药物发现.

关键词:
在PK/PD中,PK/PD是最常见的.生物分析是一种生物分析.生物标志物 生物标志物细胞 细胞 细胞 细胞 细胞适用性验证 适用性验证溶解物质的溶解物质非液体矩阵的非液体矩阵.药物动力学 药物动力学组织组织组织组织组织.

更多相关视频

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

535
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

1.9K

相关实验视频

Last Updated: May 23, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.1K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

535
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

1.9K

科学领域:

  • 药理动力学和药物新陈代谢
  • 分析化学 分析化学
  • 生物制药科学 生物制药科学

背景情况:

  • 非液态矩阵 (NLM) 对于理解药物吸收,分发,参与和药物发现和开发中的目标暴露至关重要.
  • 对NLM的定量分析对于药物开发进展至关重要.
  • 目前用于液体矩阵的生物分析实践并不直接适用于NLM,造成瓶.

研究的目的:

  • 在药物开发中建立一套全面的非液态矩阵 (NLM) 生物分析最佳实践.
  • 为了解决NLM生物分析的文献和标准化方法的缺乏.
  • 提供基于集体行业专业知识的指导.

主要方法:

  • 从整个制药行业的主题专家收集集体最佳实践的汇编.
  • 利用AAPS生物分析社区巩固行业知识.
  • 专注于解决NLM生物分析的挑战,包括不同的矩阵类型和分析严谨性.

主要成果:

  • 为NLM生物分析制定一个全面的指南.
  • 识别和减轻NLM量化所面临的具体挑战.
  • 实践的标准化,以提高药物开发效率.

结论:

  • 已建立的最佳实践将促进更强大和可靠的NLM生物分析.
  • 实施这些指导方针将有助于克服目前药物开发中的瓶.
  • 这项工作是NLM生物分析的科学家的基础资源.