缺少ATP会通过Ganoderma lucidum中的脂肪酸β-氧化途径触发甘酸的积累
Weidong Liu1, Yin Sun1, Sining Yue1
1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture; Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Microbial cell factories
|March 12, 2025
概括
低细胞内ATP水平可以促进Ganoderma lucidum的生长.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 药理学 药理学是指药理学的学科.
背景情况:
- 甘白 (Ganoderma lucidum) 生产了甘酸 (GA),这是具有显著药理潜力的三烯酸.
- 来自自然来源的低产量的天然气限制了它们的制药应用.
- 影响G. lucidum能量代谢和GA生物合成的环境因素尚不清楚.
研究的目的:
- 为了研究细胞内ATP水平在Ganoderma lucidum的甘酸 (GA) 生物合成中的作用.
- 阐明环境压力,能量代谢和GA积累之间的关系.
主要方法:
- 通过环境压力 (热量,限) 和化学处理 (甲基斯酸,酸) 诱导甘酸 (GA) 积累.
- 在各种压力条件下测量细胞内ATP水平.
- 制ATP合成酶β子单元 (ATPsyn-β) 并使用寡素 (Oli) 抑制ATP合成酶.
- 分析甘酸 (GA) 生物合成途径和乙-CoA水平.
- 研究脂肪酸β-氧化作为乙-CoA的来源.
主要成果:
- 在所有测试条件下,细胞内ATP缺乏与增加的甘酸 (GA) 积累一致相关.
- 抑制ATP合成酶 (通过基因沉默或寡素) 增加了GA并诱导了ATP缺乏.
- 缺少ATP导致了气体生物合成途径的活性增加以及乙-甲前体水平的升高.
- 强化脂肪酸β-氧化被确定为乙-CoA的主要来源,对于GA积累至关重要.
结论:
- 细胞内ATP水平是Ganoderma lucidum对环境变化的反应中的甘酸 (GA) 生物合成的关键调节因素.
- 调节ATP水平是一个可行的策略,可以提高工业生产的GA产量.
- 这项研究为优化G. lucidum种植和开发基因工程菌株以改善GA生产提供了基础.
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