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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
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与虫和牙周炎相关的微生物群:一项下一代测序研究
Euon Jung Tak1, Ok-Jin Park2, Jin-Sun Lee2
1Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, Republic of Korea.
International endodontic journal
|March 12, 2025
概括
牙顶部牙周炎的根通道中的微生物群落与牙生物膜不同. 在牙和根管中发现的特定细菌,如Hallela multisaccharivorax,与原发性顶牙牙周炎的发展有关.
科学领域:
- 微生物学 微生物学
- 牙科研究 牙科研究
- 传染性疾病 传染性疾病
背景情况:
- 牙损和牙顶部牙周炎是常见的口腔感染.
- 了解这些疾病的微生物病因对于有效治疗至关重要.
- 在原发性顶牙牙周炎中,口腔生物膜和根管微生物群之间的关系需要进一步研究.
研究的目的:
- 为了研究无牙膜生物膜的微生物群之间的相关性,牙受影响的牙生物膜,和牙的根通道在牙中与初级牙周节炎.
- 通过使用高通量测序,在不同的口腔样本地点进行细菌社区结构的比较.
主要方法:
- 从31名患者身上采集了无牙面膜 (Biofilm-C) 和牙受影响的牙 (Biofilm-E) 的生物膜样本,以及根管样本 (Canal).
- 在Illumina MiSeq平台上使用16S rRNA基因扩增序列测序分析了微生物组成.
- 统计分析包括克鲁斯卡尔-瓦利斯测试,对对变的多变量分析差异,以及MaAsLin2用于差异丰度分析.
主要成果:
- 总共有16个类,130个属和314个物种被确定.
- 在生物膜和根管样本之间观察到不同的细菌群落,没有显著的α多样性差异.
- 一些属,包括Acinetobacter,Dialister和Parvimonas,在根管样本中更为丰富. 与没有牙的牙相比,Hallella,Lactobacillus和Olsenella在牙受影响的牙中增加.
结论:
- 在根管样本和口服生物膜之间,细菌社区结构显著不同.
- 根据病状况,没有发现生物膜社区的显著差异.
- 特定的种群,如Hallela multisaccharivorax,Olsenella uli和Lactobacillus gasseri,在牙和根管中都大量存在,这表明它们在初级尖端牙周炎的发病过程中发挥了关键作用.
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