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阿佩林-13通过SIRT3/Foxo3通路减少氧化应激和线粒体功能障碍,缓解与糖尿病相关的认知衰退

Shanshan Hu¹, Chaoyang Lan¹, Shengnan Shu¹

¹Department of Neurology, Longyou County People's Hospital, Quzhou, Zhejiang, China.

Biotechnology and applied biochemistry

|March 12, 2025

概括

阿佩林-13治疗通过减少氧化应激和增强线粒体功能,改善了糖尿病小鼠的认知功能. 这种神经保护作用通过SIRT3/Foxo3通路进行介导,这表明阿佩林-13是糖尿病相关认知衰退的潜在治疗方法.

科学领域:

神经科学是一个神经科学.
内分泌学在内分泌学.
代谢障碍代谢障碍代谢障碍

背景情况:

与糖尿病相关的认知功能障碍 (DACD) 是一个日益严重的问题,临床干预有限.
阿佩林-13是一种具有已知的血管活性性质的,在神经健康方面具有潜在但不太了解的作用.
了解预防糖尿病认知衰退的机制至关重要.

研究的目的:

研究阿佩林-13对糖尿病相关认知衰退 (DACD) 的潜在保护作用.
阐明潜在的机制,包括氧化应激,线粒体功能和SIRT3/Foxo3通路.

主要方法:

一种动物模型的糖尿病被诱导使用高脂肪饮食和链条毒素.
用莫里斯水迷宫和Y迷宫测试来评估认知功能.
生物化学分析包括葡萄糖/胰岛素耐受性测试,氧化应激标志物 (SOD,CAT,MDA,4-HNE),线粒体功能测试,NAD+/NADH比率,西部斑,和ELISA,有或没有SIRT3沉默.

主要成果:

服用阿佩林-13显著改善了糖尿病小鼠的认知表现.
阿佩林-13降低了海马体的氧化应激,增强了线粒体功能,由恢复的酶活动和增加的ATP生产证明.
阿佩林-13上调SIRT3表达,增加NAD+/NADH比率,促进Foxo3a脱乙基化和核转位,在SIRT3沉默时,这些效应会减弱.

结论:

在动物模型中,Apelin-13有效地减轻了与糖尿病相关的认知衰退.
神经保护作用通过减少氧化应激和通过SIRT3/Foxo3通路改善线粒体功能来实现.
阿佩林-13代表了治疗糖尿病认知功能障碍的有希望的治疗候选者.

关键词:

在SIRT3中,SIRT3是最重要的.据报道,阿佩林13号认知功能障碍认知功能障碍糖尿病糖尿病患者糖尿病患者线粒体功能障碍线粒体功能障碍

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