相关实验视频

Updated: May 23, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

在染色体尺度上,Phyllanthus emblica L. 'Yingyu'的基因组组合

Lumin Zhang^1,2, Jianmin Yuan^1,2, Tianlei Pu^1,2

¹Tropical Eco-Agriculture Research Institute, Yunnan Academy of Agricultural Sciences, Yuanmou 651300, Yunnan, China.

DNA research : an international journal for rapid publication of reports on genes and genomes

|March 12, 2025

概括

我们向您介绍了Phyllanthus emblica,一种药用植物的第一个染色体规模的基因组. 这些基因组数据有助于识别改善水果质量和制药开发的基因.

科学领域:

基因组学就是基因组学.
植物科学植物科学
生物技术是生物技术.

背景情况:

印度 (Phyllanthus emblica L.) 是一种具有药用功能的宝贵植物.
它的遗传资源未被充分探索,限制了繁殖和利用.

研究的目的:

为了生成一个高质量的,染色体规模的基因组组合Phyllanthus emblica野生类型"Yingyu".
识别与重要特征相关的基因,如甲酸盐生物合成和组织特异性的表达模式.

主要方法:

使用全基因组测序进行新的基因组组装.
基因组注释和基因预测.
使用RNA测序进行组织特异性基因表达分析.

主要成果:

一个染色体规模的480Mb的基因组组合,包括26个伪染色体被构建,具有97.9%的基因组完整性.
有31,111个基因被注释.
在水果中确定了甲酸盐生物合成的候选基因 (GDP-曼诺斯表酶,L-银素-1,4-乳脱酶).
对鲜花中的未知功能基因和水果中的生物合成基因观察到组织特异性的表达模式.

结论:

染色体层次的基因组为Phyllanthus emblica的研究提供了宝贵的资源.
这些数据将有助于开发分子标记物,用于培育优质品种.
了解基因功能可以支持制药和营养食品应用的发展.

关键词:

菲兰塔斯 (Phyllanthus emblica) 的一个名字是L.在Hi-C的脚手架上.染色体尺度上的染色体尺度基因注释基因注释基因组组装组合的基因组.

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods

Published on: April 14, 2023

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Karyotyping

Karyotyping