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Updated: May 23, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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在活生生的微生物细胞中单分子追踪

Xiaomin Chen1, Qianhong Guo1,2, Jiexin Guan1,3

  • 1Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 611731, China.

Biophysics reports
|March 12, 2025
PubMed
概括

研究人员现在可以使用单分子成像来研究活细胞中的分子机制. 本协议详细介绍了单个粒子跟踪光激活局部化显微镜 (sptPALM) 的高分辨率细胞内分析.

关键词:
扩散系数 扩散系数活着的微生物细胞.棕树的棕树是什么意思单个分子追踪系统飞行轨迹的轨迹是可以的.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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