基于全基因组测序的物种分类,多位置测序类型和临床Aeromonas复杂的抗生素耐药性机制
Junwan Lu1, Lei Zhang2,3, Chunxia Yan1
1Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology, Jinhua, China.
Frontiers in microbiology
|March 12, 2025
概括
耐多药Aeromonas菌株含有众多β-乳糖酶基因,导致对关键抗生素的耐药性. 需要紧急监测,以控制这些重大公共卫生威胁的传播.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 耐多药性Aeromonas物种是一个日益严重的公共卫生问题.
- 这些细菌产生β-乳糖酶,使其对广泛的β-乳糖抗生素产生抵抗力.
- 了解Aeromonas抗生素耐药性机制对于有效的治疗和控制至关重要.
研究的目的:
- 为了研究临床Aeromonas分离的抗生素耐药性机制.
- 为了确定抗性基因的类型和患病率,特别是β-乳糖酶.
- 为了将基因型耐药性与表型抗生素敏感性相关联.
主要方法:
- 采集并识别了使用微扫描WalkAway和平均核酸标识 (ANI) 的78个临床Aeromonas分离物.
- 通过最小抑制度 (MIC) 测试来确定抗生素敏感性.
- 采用全基因组测序,分子克隆和生物信息分析来识别耐药性基因和移动遗传元素.
主要成果:
- 确定了7种Aeromonas物种,其中A.caviae和A.hydrophila是最常见的.
- 检测到334个抗性基因,其中β-乳糖酶占比超过55%.
- 观察到对青素和第一代头素的高耐药性,但对像卡巴烯这样的先进抗生素的耐药性较低.
结论:
- 在Aeromonas中,β-乳糖酶基因的流行与观察到的耐药性表型相关.
- 迫切需要加强对携带扩展光谱和金属β-乳糖酶基因的Aeromonas菌株的监测和控制措施.
- 这些发现凸显了多药耐药Aeromonas带来的日益严重的公共卫生威胁.
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