水性Cymbopogon citratus提取物介导的银纳米粒子:第一部分. 合成参数,表征和生物医学研究的影响
Himabindu Kurra1,2, Aditya Velidandi3, Mounika Sarvepalli3
1Department of Biotechnology, Vaagdevi Degree and P.G. College, Warangal 506001, India.
Nanomaterials (Basel, Switzerland)
|March 12, 2025
概括
使用草提取物合成银纳米粒子 (AgNPs) 的绿色合成显示出强大的抗菌和抗氧化特性. 这些环保纳米颗粒对各种生物医学应用具有前景.
科学领域:
- 纳米技术 纳米技术
- 绿色化学 绿色化学
- 生物医学科学 生物医学科学
背景情况:
- 为纳米粒子合成开发具有成本效益和环保的方法至关重要.
- 银纳米粒子 (AgNPs) 具有多样化的生物活动,但它们的合成往往涉及恶劣的化学物质.
- 草 (Cymbopogon citratus) 是一种随时可用的植物,具有潜在的植物化学还原剂.
研究的目的:
- 为了探索银纳米粒子 (AgNPs) 的绿色合成,利用Cymbopogon citratus提取物.
- 描述合成的AgNP并评估它们的物理化学性质.
- 研究这些绿色合成的AgNP的潜在生物医学应用,包括抗菌,抗炎,抗糖尿病,抗氧化和细胞毒性活动.
主要方法:
- 在最佳条件下使用银酸盐和Cymbopogon citratus提取物 (1.50mM AgNO3,3.5%v/v提取物,100°C,pH9.60分钟) 的AgNP的绿色合成.
- 使用技术来确定AgNP的形态,水力动力直径,泽塔电位和晶体结构的特征.
- 通过区域抑制试验评估抗菌活性.
- 在体外评估抗炎,抗糖尿病 (α-葡萄糖酶和α-氨基酶抑制) 和抗氧化活性.
- 对癌症和非癌症细胞系的细胞毒性测定和生态毒性研究.
主要成果:
- 通过颜色变化证实了AgNPs的成功绿色合成,其特点是球形形态,水力动力直径为135.41 ± 49.30nm,以及29.9 ± 1.4mV的泽塔电位.
- 观察到显著的抗菌活性,在度超过20μg/井时有效性增加.
- 已在体外证明具有抗炎,抗糖尿病 (α-葡萄糖酶和α-氨基酶抑制) 和强烈的抗氧化活性.
- 与多克索鲁比相比,AgNPs的细胞毒性较低,对细胞的作用取决于度.
- 生态毒性测定表明急性毒性较低,但长期暴露可能带来潜在风险.
结论:
- 使用Cymbopogon citratus提取物进行AgNPs的绿色合成是可行的,并产生稳定的纳米粒子.
- 合成的AgNP具有显著的抗菌,抗炎,抗糖尿病和抗氧化特性.
- 这些环保的AgNP为生物医学应用提供了有希望的替代方案,需要进一步研究它们的治疗潜力和安全性.
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