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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: May 22, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

体积测量,微流体和等离子RT-PCR.

Harshit Harpaldas Chellani¹, Kelia Human¹, Robert Stanciu¹

¹Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.

|March 12, 2025

概括

此摘要是机器生成的。

这项研究引入了一种快速的微流体等离体逆转录 (RT) -PCR方法,用于分散的病原体检测. 它使用光激活纳米粒子和闭环温度控制在短短16分钟内实现了SARS-CoV-2放大.

关键词:

微流体学在微流体学方面病原体检测检测病原体的检测血性PCR的使用方法医疗保健诊断诊断的点的护理.

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Microfluidic Chip Fabrication and Method to Detect Influenza

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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

相关实验视频

Last Updated: May 22, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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科学领域:

生物技术是生物技术.
纳米技术纳米技术
分子诊断学分子诊断

背景情况:

病原体的分散分子检测对公共卫生至关重要.
传统的聚合酶连锁反应 (PCR) 热循环在临床环境中面临着挑战.
使用光激活纳米材料的等离子PCR为快速热循环提供了一个有希望的替代方案.

研究的目的:

开发和展示一个体积,微流体等离子体逆转录 (RT) -PCR方法.
集成闭环温度控制以提高精度和速度.
为了实现快速,紧和分散的病原体检测.

主要方法:

制造一个带有集成热电偶的微流体芯片,用于实时温度监测.
使用红外LED实现比例积分导数 (PID) 算法,用于闭环温度控制.
散金纳米棒溶液与RT-PCR试剂用于光诱导加热.
开发一个包含红外LED,风扇和度仪的仪器,用于等离子RT-PCR和检测.

主要成果:

在16分钟内实现了SARS-CoV-2的快速热循环和放大 (5分钟RT,11分钟45个周期).
在具有精确温度控制的微流体芯片中证明了体积等离子PCR.
验证了集成热电偶和PID控制对快速热循环的有效性.

结论:

开发的微流体等离子RT-PCR系统能够显著更快地检测病原体.
这种方法对快速,紧和分散的分子诊断具有前景.
微流体和纳米材料的整合是推动护理点分子测试的关键.