磁性向传递益生菌以控制其在肠道中的停留和积累
Hanye Xing1, Xingyu Liu1, Ju Wang1
1Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. songyh@hfut.edu.cn.
这项研究引入了磁控益生菌 (LGG@P@Fe3O4),以改善治疗胃肠道疾病的肠道保留. 磁性向显著提高益生菌的积累和居住在肠道.
科学领域:
- 生物材料科学 生物材料科学
- 胃肠病学 胃肠病学
- 微生物学 微生物学
背景情况:
- 口服益生菌在治疗胃肠道疾病的有效性有限,原因是肠道保留不良.
- 开发增强益生菌输送和肠道居住的策略对于改善治疗结果至关重要.
研究的目的:
- 开发和评估一个磁控制的益生菌输送系统,以提高肠道积累和保留.
- 在模拟的胃肠道条件和体内环境中评估工程益生菌的稳定性,活力和磁性反应.
主要方法:
- 通过通过静电自组装将氨基基改性氧化铁纳米颗粒 (Fe3O4-NH3+NP) 连接到聚多巴胺涂层的Lacticaseibacillus rhamnosus GG (LGG@P) 来制造磁性益生菌 (LGG@P@Fe3O4).
- 在模拟的胃肠道环境中评估了结构稳定性和益生菌活力.
- 在小鼠模型中使用体外光密度测量和体内肠道光成像来评估磁性操纵,积累和保留.
主要成果:
- 在模拟的胃肠道条件下,LGG@P@Fe3O4表现出结构稳定性和益生菌活力.
- 外部磁场在体外显著增强了益生菌的积累和保留,将悬浮的光密度从约1.17降低到约0.29.
- 在小鼠体内研究显示,在24小时后接触磁铁的小组中,肠道光显著更强,这表明肠道保留改善.
结论:
- 磁控益生菌输送策略 (LGG@P@Fe3O4) 是一种可行且易于制造的方法.
- 这种方法有效地提高了益生菌在肠道中的积累和居住,为改善胃肠道疾病的治疗提供了一个有前途的战略.
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