通过ipsilateral与contralateral免疫对SARS-CoV-2进行生殖中心和抗体动态的调节
Lauren Burmas1, Wen Shi Lee1, Andrew Kelly1
1Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
保持相同的接种部位 (双侧) 可以更快地提高抗体标位,但切换部位 (对侧) 对抗体对SARS-CoV-2变种的免疫力没有长期影响. 这两种策略随着时间的推移产生了可比的结果.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 病毒学 病毒学
背景情况:
- 临床试验表明,在ipsilateral (同一侧) 和contralateral (替代侧) 主助力疫苗接种策略之间,免疫学结果不同.
- 关于解剖部位选择如何影响生殖中心 (GC) 和疫苗接种后抗体反应的机制理解有限.
研究的目的:
- 在小鼠中使用辅助的SARS-CoV-2尖端疫苗进行ipsilateral与contralateral主要增强疫苗接种后剖析生殖中心 (GC) 动态和血清学结果.
- 阐明疫苗接种部位定位对B细胞记忆和抗体反应的影响,包括对SARS-CoV-2变种的交叉反应.
主要方法:
- 使用C57BL/6小鼠模型与辅助的SARS-CoV-2尖端疫苗.
- 接种了主要增强疫苗,可以是ipsilaterally或contralaterally.
- 分析了生殖中心 (GC) 形成和淋巴结排水的动态,以及血清抗体标位和对抗SARS-CoV-2变种 (野生型,Beta,BA.1) 的中和能力.
主要成果:
- 反侧面疫苗接种导致独立的GC,二次GC只在远端再接种时形成,而初级GC没有增强.
- 双侧疫苗接种维持了GC活性,加速了对祖先 (WT),Beta和BA.1变体的抗体标位的发展.
- 疫苗接种后28天后,抗体的大小,耐用性和中和能力在ipsilateral和contralateral组之间是可比的. 交叉反应性GCB细胞显示基于疫苗接种方案的差异性抗原识别和细分.
结论:
- 保持一个共同的主要增强位点 (二侧位) 增强了B细胞回忆的动力学,并暂时增加了抗体标位.
- 长期的血清学结果,包括抗体大小,耐用性和对抗SARS-CoV-2变体的中和能力,最终不受免疫的解剖本地化影响.
- 疫苗接种地点策略会影响动力学,但不会影响适应性免疫反应的总体规模或持久性.
更多相关视频
08:48Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Published on: February 16, 2022
06:15Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Published on: September 7, 2018
相关概念视频
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
Humoral Immune Responses
Cells of the Adaptive Immune Response
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
