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Updated: May 22, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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通过OMEGA引导的DNA聚合酶,可以在可调节的窗口中实现随机突变

Li-Hua Liu1, Wei Lei2, Zhiqian Zhang2

  • 1Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong 510000, PR China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou 515063, PR China.

Trends in biotechnology
|March 12, 2025
PubMed
概括

我们开发了OMEGA-R,这是一种新的有针对性的随机突变发生系统,可以显著提高目标突变率,并最大限度地减少目标外影响. 这种高效的工具增强了繁殖,定向进化和基因功能研究.

关键词:
在 PolI3M-TBD-SpyTag 中使用.在SpyCatcher中使用IscBB.指导进化是指导进化的.有针对性的随机突变发生.

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