盐应激激活了CDK8-AHL10-SUVH2/9模块,以动态调节Arabidopsis中的盐耐受性
Pengcheng Guo1, Leelyn Chong1, Zhixin Jiao1
1The Zhongzhou Laboratory for Integrative Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Nature communications
|March 13, 2025
概括
介质环素依赖酶8 (CDK8) 活性在盐应激下增加,通过降解AT动机核局部化蛋白10 (AHL10) 来促进植物盐耐受性. 这种CDK8-AHL10通路控制了对盐的敏感基因.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 盐应激严重影响农业,需要了解植物对盐的耐受机制.
- 植物中对盐敏感基因转录的关键调节者在很大程度上是未知的.
研究的目的:
- 为了确定植物中盐分耐受性的关键调节剂.
- 阐明控制盐反应基因表达的分子机制.
主要方法:
- 研究了中介环林依赖激酶8 (CDK8) 在盐耐受性中的作用.
- 利用蛋白组学来识别CDK8酸化标,包括AT动机核局部化蛋白10 (AHL10).
- 进行了转录组分析和染色体免疫沉,以了解基因调节.
主要成果:
- 盐应激诱导CDK8激酶活性,增强盐耐受性.
- CDK8酸化AHL10,促进其降解,并对盐分耐受性产生负面影响.
- CDK8-AHL10模块调节超过20%的盐反应基因,其中AHL10招募SUVH2/9通过H3K9me2沉积抑制基因表达.
结论:
- 确定了CDK8-AHL10-SUVH2/9模块作为植物盐耐受性的关键调节器.
- 该模块作为一个分子开关,在盐应力过程中控制转录动态.
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