相关实验视频
Updated: May 22, 2025
13:07
One-channel Cell-attached Patch-clamp Recording
Published on: June 9, 2014
24.2K
Piezo1通过激活Ca2(+) /CaMKII依赖途径促进死性肠球炎的进展
Zhenyu Li1,2, Qinlei Jiang1,2, Jiaqi Wei1,2
1Department of Neonatology, Children's Medical Center, The First Hospital of Jilin University, Changchun, Jilin, China.
Communications biology
|March 13, 2025
概括
死性肠球炎 (NEC) 涉及Piezo1,一个在肠细胞中的机械传感器. 在小鼠中阻断Piezo1通过调节屏障功能和细胞因子释放来减少NEC炎症.
科学领域:
- 胃肠病学 胃肠病学
- 新生儿科学 新生儿科学
- 细胞生物学 细胞生物学
背景情况:
- 结核性肠球炎 (NEC) 是早产婴儿的严重肠道疾病,治疗选择很少.
- 肠上皮细胞 (IEC) 中的一种机械传感器Piezo1与肠道炎症有关,但其在NEC中的作用尚不清楚.
研究的目的:
- 调查Piezo1在NEC发展中的作用.
- 阐明Piezo1对NEC病原发生的分子机制.
主要方法:
- 在不同妊娠年龄的早产婴儿中比较Piezo1表达.
- 使用维林特异的Piezo1敲击小鼠诱导和研究NEC.
- 研究了Piezo1在水平,CaMKII和NF-κB信号传导上的作用.
- 在NEC模型中评估了一种CaMKII抑制剂 (KN93) 的治疗潜力.
主要成果:
- 在妊娠年龄较低的早产婴儿中,Piezo1的表达更高.
- 在IECs中的Piezo1淘汰赛改善了肠道屏障功能,减少了细胞因子分泌,并在NEC小鼠模型中减少了炎症.
- 皮埃佐1激活增加了细胞质,促进了CaMKII/NF-κB相互作用和激活.
- 在体外和体内的IEC中,KN93部分抑制了Piezo1的功能.
结论:
- Piezo1在NEC的发展中发挥着重要作用.
- Piezo1通过信号传导,CaMKII和NF-κB激活,为NEC病变产生贡献.
- 针对Piezo1或像CaMKII这样的下游途径可能为NEC提供治疗策略.
相关概念视频
01:16Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
01:12Mechanically-gated Ion Channels
6.2K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.2K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.0K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K