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在上皮细胞粘附分子介导循环瘤细胞隔离的多方面的方法

Dora Szerenyi¹, Gabor Jarvas^1,2, Andras Guttman^1,2,3

¹Research Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, 8200 Veszprem, Hungary.

Molecules (Basel, Switzerland)

|March 13, 2025

概括

循环瘤细胞 (CTC) 对转移至关重要. 基于上皮细胞粘附分子 (EpCAM) 的捕获受到细胞异质性和上皮细胞转移到介质酶的限制,因此需要替代生物标志物来改善癌症管理.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物标志物发现发现

背景情况:

循环瘤细胞 (CTC) 是癌症转移的关键指标,对监测疾病进展至关重要.
目前的方法通常依赖于上皮细胞粘附分子 (EpCAM) 来分离CTC.
然而,瘤细胞异质性和上皮转移到介质酶转移 (EMT) 现象可能会降低EpCAM表达,阻碍捕获效率.

研究的目的:

批判性地审查 EpCAM 在 CTC 捕获中的作用.
探索EMT对EpCAM表达和CTC隔离的影响.
讨论替代生物标志物和提高液体活检中CTC检测的策略.

主要方法:

对CTC捕获技术现有研究的文献综述.
对表皮细胞转变为介质细胞对EpCAM表达的影响的分析.
评估替代生物标志物和新型隔离策略.

主要成果:

在表皮细胞转化为介质细胞的过程中,EpCAM表达显著改变,限制了其作为通用CTC标记物的实用性.
在CTC之间的异质性进一步复杂化了依赖EpCAM的隔离.
替代标记和多标记方法对更广泛的CTC检测有希望.

结论:

基于EpCAM的CTC捕获由于EMT和细胞异质性而不足.
开发新的,独立于EpCAM的策略对于全面的液体活检至关重要.
改善CTC隔离将提高癌症诊断,预后和治疗监测.

关键词:

在CTC中,使用CTC.在EMT中,EMT是EMT.免疫亲和关系的免疫亲和关系没有标签的隔离.液体活检活检液体活检表面标记器表面标记器

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