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Updated: May 22, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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Published on: November 7, 2012

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通过基于计算的理性设计提高Bacillus licheniformis性蛋白酶2709的热稳定性

Yuan Yuan1, Guowei Zhao1, Jing Lu2

  • 1College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

Molecules (Basel, Switzerland)
|March 13, 2025
PubMed
概括

工程性蛋白酶 (AprE 2709) 通过向突变显示出更好的热稳定性和活性. 这提高了其在高温工艺中的工业应用潜力.

关键词:
性蛋白质酶是一种性蛋白质酶.脱化反应的脱化反应合理的设计理性的设计.热稳定性的热稳定性

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For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
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The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
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Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
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