乳酸的原生和重组酵母生产者:特征和前景
Aksyniia Tsaruk1,2, Kamila Filip1, Andriy Sibirny1,3
1Faculty of Biotechnology, Collegium Medicum, University of Rzeszow, 35-601 Rzeszow, Poland.
International journal of molecular sciences
|March 13, 2025
概括
像Saccharomyces cerevisiae这样的酵母由于其独特的特征,对乳酸 (LA) 生产有希望. 这些酵母的工程设计为可再生生物塑料和化学品提供了可持续的循环经济解决方案.
科学领域:
- 生物技术和工业微生物学
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 乳酸 (LA) 是一种重要的工业化学物质,传统上由乳酸细菌生产.
- 酵母与细菌相比具有优势,包括增强的酸耐受性,更广泛的基质利用,以及更大的基因操纵潜力.
- 几种酵母菌种正在被探索为各种工业应用的高效LA生产者.
研究的目的:
- 审查特定酵母菌株 (Lachancea thermotolerans,Saccharomyces cerevisiae,Kluyveromyces marxianus,Kluyveromyces lactis,Candida utilis,Pichia kudriavzevii) 在乳酸生产方面的潜力.
- 突出每个酵母菌种的独特特性和工业适用性.
- 讨论克服挑战和推进基于酵母的LA生物合成的战略.
主要方法:
- 对基于酵母的乳酸生产现有文献的审查.
- 酵母特性分析,包括耐酸性,基质范围和应力弹性.
- 探索代谢工程策略,如异质乳酸脱酶 (LDH) 表达和适应性实验室进化.
主要成果:
- 麦芽提供了一个强大的遗传系统和耐酸性.
- 克劳维罗米切斯马克西南斯 (Kluyveromyces marxianus) 具有耐热性和高效的粉/乳糖利用.
- 其他物种,如Kluyveromyces lactis,Pichia kudriavzevii和Candida utilis,对特定应用,如乳制品废物回收利用和生物炼油厂,显示出希望.
结论:
- 工程酵母代表了工业乳酸生产的可持续替代品,与循环经济原则保持一致.
- 需要进一步的研究来优化代谢途径,增强LA耐受性,并开发可扩展的生物过程.
- 基于酵母的LA生产可以帮助满足对可再生和可生物降解材料的需求.
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