在Oreochromis niloticus中的负选择 × O. 混合体表明不兼容的氧化酸化 (OXPHOS) 蛋白质
Andrey Shirak1, Arie Yehuda Curzon1,2, Eyal Seroussi1
1Institute of Animal Science, Agricultural Research Organization, Rishon LeTsiyon 75288, Israel.
International journal of molecular sciences
|March 13, 2025
概括
混合鱼繁殖的目标是所有雄性后代,这对水产养殖至关重要. 研究人员在线粒体氧化酸化 (OXPHOS) 蛋白中发现了细胞核不相容性,揭示了Oreochromis niloticus和Oreochromis aureus物种之间的繁殖障碍.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 遗传学 遗传学 是一个
- 进化生物学 进化生物学
背景情况:
- 混合鱼 (Oreochromis niloticus x Oreochromis aureus) 生产全男性后代,对水产养殖至关重要,但受到了生殖障碍的阻碍.
- 逃跑的杂交物面临环境压力因素,使它们的适应性和遗传兼容性成为一个关键的研究领域.
- 线粒体氧化酸化 (OXPHOS) 复合体,涉及线粒体 (mtDNA) 和核 (nDNA) DNA,对于能源生产和混合适应至关重要.
研究的目的:
- 研究Oreochromis niloticus (On) 和Oreochromis aureus (Oa) 之间的OXPHOS蛋白质中的细胞核不相容性.
- 开发特定物种的探测器,以确定杂交鱼的起源.
- 评估这些不兼容性对混合体适应性和生殖成功的影响.
主要方法:
- 在On和Oa之间对116个OXPHOS蛋白进行比较分析,以确定未保存的氨基酸替代物.
- 基于鉴定出的遗传差异,开发了13种特定物种的探针.
- 对162个序列读取档案 (SRA) 实验进行选,以检测杂交起源和mtDNA-nDNA等位基因不匹配.
主要成果:
- 39.5%的选SRA实验表明混合起源与mtDNA-nDNA等位基因不匹配.
- 对于UQCRC2,ATP5C1和COX4B,观察到跨种类mtDNA-nDNA等位基因组合的频率明显低于预期.
- 这些低频率表明负选择和细胞核不相容性,有助于生殖障碍.
结论:
- 在OXPHOS基因中的细胞核不相容性代表了Oreochromis niloticus和Oreochromis aureus之间的显著生殖障碍.
- 这些发现为了解杂交鱼的局限性和潜在的生态影响提供了分子基础.
- 这项研究提供了关于驱动鱼类生殖隔离的进化机制的见解.
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