信号是一种通用碳源信号转换器,并对过去的碳源产生离子记忆
Kobi Simpson-Lavy1, Martin Kupiec1
1The Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
International journal of molecular sciences
|March 13, 2025
概括
细胞通过使用一种通用机制,适应基因表达的各种碳来源. 葡萄糖代谢物如葡萄糖-6-酸盐 (G6P) 和葡萄糖-1-酸盐 (G1P) 触发信号,影响基因调节并产生"离子记忆".
科学领域:
- 细胞代谢的细胞代谢.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞优先利用葡萄糖,但可以适应其他碳来源.
- 除了催化剂抑制之外,将代谢基因表达与各种碳来源相匹配的精确机制尚未完全理解.
研究的目的:
- 研究细胞识别和适应不同碳源的机制.
- 阐明特定代谢物和信号通路在代谢基因调节中的作用.
主要方法:
- 研究了七种不同的碳源对Snf1酸化和下游基因表达的影响.
- 分析了糖解代谢物葡萄糖-6-酸盐 (G6P) 和葡萄糖-1-酸盐 (G1P) 在信号传输中的作用.
- 研究了涉及Pma1,calcineurin和Cmk1.1的信号通路.
主要成果:
- G1P与G6P的比率通过Pma1激活信号,调节ADH2和ZWF1.1等基因.
- 氨酸信号减少了ADH2的表达,而Cmk1信号增加了ZWF1的表达.
- 证明了"离子记忆",过去的碳来源会影响当前的信号和基因表达.
- 通过使用银河糖成功操纵了这种机制,以实现高ZWF1表达.
结论:
- 确定了细胞对所有碳来源的反应的通用机制,以G1P/G6P比率和信号为中心.
- 这种机制通过"离子记忆"将当前的碳来源信息与过去的环境线索集成在一起.
- 提供了对代谢适应和基因调节的新见解,超出了简单的催化剂抑制.
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