Jove
Visualize
联系我们

相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

685
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
685
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.3K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ligation-Driven Electrochemical Magneto-Genoassay Platform Based on PNA Probes for the Multiple Detection of Soy and Mustard DNA in Wheat Flour.

Biosensors·2026
Same author

Determination of Histamine in Tuna Fish by a Rapid and Green DART-TQ MS Method.

Journal of mass spectrometry : JMS·2026
Same author

Metabolomic Profiling of Commercial Tomato Puree by One-Shot Mass Spectrometry-Based Analysis: A Qualitative Perspective.

Metabolites·2025
Same author

Whey Proteins and Bioactive Peptides: Advances in Production, Selection and Bioactivity Profiling.

Biomedicines·2025
Same author

DART-Triple Quadrupole Mass Spectrometry Method for Multi-Target and Fast Detection of Adulterants in Saffron.

Metabolites·2025
Same author

Unraveling the Biological Properties of Whey Peptides and Their Role as Emerging Therapeutics in Immune Tolerance.

Nutrients·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: May 22, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.3K

基于质谱的多种过敏原控制方法:适用于面包制品

Anna Luparelli1, Elisabetta De Angelis1, Rosa Pilolli1

  • 1Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Via G. Amendola, 122/O, 70126 Bari, Italy.

Foods (Basel, Switzerland)
|March 13, 2025
PubMed
概括

这项研究验证了一种质谱法,用于检测加工烤制品中的多种食物过敏原,如蛋,牛奶和大豆. 该方法可靠地识别微量过敏原,即使在广泛的食品加工后,提高消费者保护.

关键词:
面包制品 面包制品 面包制品食物过敏原 食物过敏原食品加工 食品加工 食品加工隐藏的过敏原是隐藏的高分辨率的质谱学高分辨率质谱学.多目标方法多目标方法.

更多相关视频

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

535
Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
10:24

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

1.0K

相关实验视频

Last Updated: May 22, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.3K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

535
Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
10:24

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

1.0K

科学领域:

  • 食品科学 食品科学 食品科学
  • 分析化学 分析化学
  • 质谱测量质量谱测量

背景情况:

  • 食品加工可以改变过敏原的结构,使检测复杂化.
  • 准确的过敏原量化对于保护过敏消费者至关重要.
  • 质谱法 (MS) 是确定过敏原的关键确认方法.

研究的目的:

  • 研究一种多过敏原MS/MS方法的适用性,用于检测加工饼干和饼中的微量过敏原.
  • 为了验证蛋白型标记物用于追踪制品中的过敏原,尽管进行了热和技术处理.
  • 评估该方法在不同MS平台 (低分辨率和高分辨率) 的稳定性.

主要方法:

  • 利用ThrAll项目中开发的多种过敏原MS/MS方法.
  • 应用该方法检测蛋,牛奶,大豆,杏仁,子,花生和芝麻在饼干和饼干中.
  • 将方法从低分辨率转移到高分辨率的MS系统进行验证.
  • 计算的检测极限 (LOD) 和量化极限 (LOQ).

主要成果:

  • 多种过敏原MS/MS方法在经过大量加工的饼干和饼干中成功检测到微量过敏原.
  • 经过验证的蛋白型标记物在追踪过敏原方面被证明是有效的,尽管加工条件不同.
  • 该方法证明了在低分辨率和高分辨率MS平台上的稳定性和适用性.
  • 进行了灵敏度分析 (LOD/LOQ),并调查了加工对过敏原检测的影响.

结论:

  • 经过验证的多过敏原MS/MS方法适用于在具有挑战性的制食品矩阵中检测微量过敏原.
  • 已识别的标记物是强大的,可以在不同的MS平台上可靠地使用.
  • 这种方法提高了确保食品安全的能力,并保护过敏个体免受意外的过敏原暴露.