综合代谢学和转录学分析确定了SMN-LBK中Lacticaseibacillus paracasei中的关键氨基酸代谢机制
Jie Shen1,2,3, Yuyu Du1,2,3, Yuechenfei Shen1,2,3
1Key Laboratory of Agricultural Product Processing and Quality Control of Specialty (Co-Construction by Ministry and Province), School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
Foods (Basel, Switzerland)
|March 13, 2025
概括
缺少谷氨酸和氨酸会抑制乳酸糖菌的生长. 用α-谷氨酸 (一种谷氨酸前体) 补充剂逆转了这种抑制,揭示了乳酸细菌发酵中受到影响的关键代谢途径.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 乳酸菌发酵在食品工业中至关重要.
- 发酵基质的蛋白质成分会影响产品的特性.
- 乳酸细菌 (LAB) 中的氨基酸代谢对于食品应用至关重要.
研究的目的:
- 调查关键氨基酸缺陷对乳酸乳酸菌 (Lacticaseibacillus paracasei) SMN-LBK生长的影响.
- 阐明这些缺陷背后的代谢机制.
- 为优化LAB发酵提供理论基础.
主要方法:
- 基因组分析和体外添加试验.
- 代谢学和转录学分析.
- 评估生长抑制和代谢途径的改变.
主要成果:
- 谷氨酸和氨酸缺陷显著抑制了SMN-LBK的生长 (p < 0.05).
- 阿尔法谷氨酸 (一种谷氨酸前体) 的添加挽救了生长抑制.
- 缺陷影响了碳水化合物和氨基酸代谢,包括酸途径和糖解/葡萄糖生成.
结论:
- 谷氨酸和氨酸对于乳糖菌菌SMN-LBK的生长至关重要.
- 阿尔法谷氨酸补充剂可以减轻生长抑制.
- 了解氨基酸代谢是控制食品应用中LAB发酵的关键.
关键词:
乳酸酶ibacillus paracasei SMN-LBK 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei 乳酸酶ibacillus paracasei在阿根廷,阿根廷人.谷氨酸酸盐的使用方法这些机制的机制.相关概念视频
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