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冷后发酵过程对蛋白酶辅助发酵苏中的微生物多样性和生物氨基的影响
Xiaogang Guo1, Kaiyi Chen1, Li Chen1
1School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, China.
Foods (Basel, Switzerland)
|March 13, 2025
概括
酶辅助的低温发酵通过促进微生物多样性和酶活性来提高苏夫质量. 这种方法有效地应对冷发酵的挑战,影响生物氨基的形成和产品的整体特性.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 传统的苏夫发酵在低温下面临挑战,可能会影响微生物动态和产品质量.
- 了解微生物群落,酶活性和物理化学变化之间的相互作用对于优化苏夫生产至关重要.
研究的目的:
- 研究由酶辅助的低温冷发酵对苏福微生物多样性,生物氨基 (BA) 形成和物理化学性质的影响.
- 将低温酶辅助发酵 (LTEF20) 与室温发酵 (RTEF30) 和非酶发酵 (RTNF30) 进行比较.
主要方法:
- 在室温和低温下对苏夫进行了酶辅助发酵.
- 分析包括微生物多样性,生物氨基水平,总酸 (TA),氨基 (NH3-N) 和酶活性.
- 进行了相关性分析,以将物理化学特性与微生物群落联系起来.
主要成果:
- 与RTNF30相比,酶辅助发酵 (LTEF20和RTEF30) 显著增加了TA,NH3-N和酶活性.
- 在LTEF20组显示最高的总BA (3.7 mg/g) 和素 (1.8 mg/g).
- LTEF20表现出更高的微生物多样性,含有高水平的肠杆菌和乳酸细菌.
结论:
- 酶辅助的低温发酵有效地克服了苏福生产的挑战,提高了关键的质量参数.
- 在低温发酵下微生物活动的变化显著影响了苏福的发酵过程和质量.
- 特定的微生物相关性,如布特雷辛与Bifidobacterium和TA与Enterococcus,突出了未来研究生物氨基调节的途径.
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