乌尔索利克酸诱导多方面的防御反应对收割后的蓝色菌腐烂在果果的果实
Chang Shu1, Wenxiao Jiao2, Kuanbo Cui3
1College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods (Basel, Switzerland)
|March 13, 2025
概括
乌尔索酸 (UA) 通过抑制病原体生长,对果蓝菌具有抗真菌特性. 它还激活了果的果实.
科学领域:
- 植物病理学 植物病理学
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
背景情况:
- 由Penicillium expansum引起的果收获后腐烂导致了重大的经济损失.
- 了解植物防御反应的分子机制对于开发有效的疾病控制策略至关重要.
研究的目的:
- 调查果果中的乌尔索酸 (UA) 诱导的抗病和防御机制,以对抗P. 扩张金额. 扩张金额
- 评估UA作为控制收获后蓝色菌腐烂的诱因的潜力.
主要方法:
- 在体外抗真菌活性测定UA对P. 扩张金额. 扩张金额
- 在UA溶液中浸泡果果,然后进行病原体注射.
- 酶活性测定 (酸酶,β-1,3-葡萄糖酶,过氧化酶,多氧化酶,氨氨酸氨基酶,酸-4-氧化酶,4-酸辅酶A结合酶,超氧化物脱酶,酸盐过氧化酶,谷氨缩小酶,催化酶).
- 测量总化合物,黄类和素含量的测量.
主要成果:
- 乌尔索酸表现出固有的抗真菌活性,抑制P. 在体外 (in vitro) 进行膨胀生长和子发芽.
- 用UA治疗显著增强了果的抗病能力.
- 氨酸诱导了多方面的防御机制,包括防御和抗氧化酶的激活以及烯胺路径.
- 观察到奇丁酶,β-1,3-葡萄糖酶,过氧化酶,多氧化酶和烯胺通路酶的活性增加.
- 治疗UA导致总,黄和素的积累增加.
- 抗氧化酶活动 (SOD,APX,GR) 被增强,而催化酶活动被抑制,调节过氧化水平.
结论:
- 乌尔索酸有效地诱导果果对收获后蓝色菌腐烂的抗病能力.
- 防御机制包括水解酶的激活,烯胺路径和氧化应激的调节.
- 乌尔索酸作为一种自然诱导剂,可以增强水果的防御能力和控制收获后腐烂.
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