在牛奶中Bacillus cereus的基因组和转录基因组分析:对甜甜的曲缺陷的洞察
Maria Kyritsi1, George Tsiolas1,2, Antiopi Tsoureki1
1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 57001 Thessaloniki, Greece.
Foods (Basel, Switzerland)
|March 13, 2025
概括
细菌谷物菌会导致牛奶甜,这是一个影响乳制品的缺陷. 这项研究确定了在化过程中调高的特定基因,为早期坏检测和控制提供了目标.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 细菌大麦肉味大 (B. cereus s.l.) 是一种植物. 是常见的食物传播微生物.
- B. cereus会导致腐烂缺陷,如牛奶中的甜,影响乳制品.
- 不过,B. cereus诱导的甜的遗传基础尚不清楚.
研究的目的:
- 为了研究B. cereus诱导的甜背后的遗传和功能机制.
- 在牛奶腐烂过程中分析新分离的B. cereus菌株 (BC46) 的基因组和转录组.
主要方法:
- 使用Illumina和Nanopore测序对B. cereus菌株BC46的全基因组测序.
- 在牛奶中的两个生长阶段对BC46的转录组测序.
- 对基因组和转录基因组数据的生物信息分析.
主要成果:
- 该BC46基因组组装为5.6Mb,GC含量为35.1%,将其分类为B.cereus sensu stricto (B.cereus s.s.).
- 鉴定出病毒性因素,抗微生物耐药性基因和感冒冲击蛋白.
- 参与胞形成,毒素产生,水解和蛋白质溶解的基因在表现出甜的牛奶样本中显著上调.
结论:
- 在B. cereus BC46中,特定的上调基因提供了关于乳糖的分子基础的见解.
- 这些发现可以帮助开发用于早期检测乳制品腐败的遗传策略.
更多相关视频
相关概念视频
Ribosome Profiling
3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K
Genomics
35.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.6K
RNA-seq
9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Proteomics
7.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.1K
DNA Microarrays
17.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.1K


