通过调节Ganoderma lucidum中的反应性氧物种,AOX影响了多糖的合成
Ruiying Zhu1, Lele Zhang1, Longxi Wu1
1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
Foods (Basel, Switzerland)
|March 13, 2025
概括
替代氧化酶 (AOX) 调节活性氧物种 (ROS) 并影响 Ganoderma lucidum 中的多糖化物产生. 操纵AOX水平会改变ROS和抗氧化剂基因表达,影响多糖化合物合成和抗氧化剂活性.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 替代氧化酶 (AOX) 是一种线粒体终端氧化酶,对于平衡活性氧物种 (ROS) 和氧化还原稳定至关重要.
- 了解AOX的作用对于真菌的代谢调节至关重要.
研究的目的:
- 为了研究AOX在Ganoderma lucidum中的功能.
- 为了确定AOX对ROS产生,抗氧化基因表达和多糖化合物合成的影响.
主要方法:
- 构造的AOX沉默 (AOXi) 和AOX过度表达 (OE-AOX) 菌株的Ganoderma lucidum.
- 量化ROS水平和抗氧化基因 (pgm, pmi) 转录.
- 提取,净化和表征细胞外多糖 (EPSs).
- 评估了EPSs的抗氧化活性.
主要成果:
- AOX静止高调的ROS和抗氧化基因表达,增加多糖的产量约38% (AOXi25).
- 过度表达AOX降低了ROS和抗氧化基因表达的调节,降低了多糖的产量约80% (OE-AOX21).
- 来自不同菌株的EPS在单糖化合物组成和分子量方面有所不同.
- 所有EPS都显示出自由基清除活性,AOXi25的酸性EPS是最有效的.
结论:
- 由AOX衍生的ROS显著调节了Ganoderma lucidum中的多糖化合物合成和积累.
- 在G. lucidum中,AOX是调节EPS生产和抗氧化性质的关键目标.
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