相关实验视频

Updated: May 22, 2025

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

细胞外Z-DNA增强生物膜的空心性

S L Han^1,2, J Wang^1,2, H S Wang¹

¹State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Journal of dental research

|March 13, 2025

概括

细胞外Z-DNA是一种左撇子形式的DNA,在牙生物膜中普遍存在. 它的增加形成增强了生物膜的稳定性和酸的产生,有助于牙.

科学领域:

微生物学微生物学
分子生物学分子生物学
牙科研究牙科研究

背景情况:

细胞外DNA (eDNA) 在生物膜结构和微生物附着中至关重要.
在牙病原性中,eDNA构造多样性的作用,特别是B-DNA到Z-DNA的转换,在很大程度上是未知的.
牙相关的生物膜是复杂的多物种环境,eDNA功能需要进一步研究.

研究的目的:

为了研究在空心生物膜中细胞外Z-DNA的存在和生物功能.
为了确定Z-DNA形成对生物膜结构稳定性和焦化性的影响.
探索针对细胞外Z-DNA的潜在抗生物膜策略.

主要方法:

在各种组织和生物膜中检测和量化细胞外Z-DNA,包括那些由*Streptococcus mutans*主导的组织和生物膜.
对细胞外Z-DNA含量的物种特异性的分析.
调节Z-DNA形成并评估其对细胞外多糖水平,DNase耐药性,结构密度和酸性生产的影响.

主要成果:

细胞外Z-DNA广泛存在于各种组织和菌生物膜中,特别是那些涉及*Streptococcus mutans*的生物膜中.
细胞外Z-DNA含量表现出特定物种的变异.
增加的Z-DNA形成通过改善DNase耐药性,结构密度和酸性产生,显著提高生物膜化性,同时也影响细胞外多糖水平.

结论:

细胞外Z-DNA在牙损伤的发生和活动中起着重要作用.
电子DNA向Z-DNA的构造过渡增强了化生物膜的特性.
向细胞外Z-DNA为抗生物膜治疗牙损伤提供了一个潜在的新战略.

关键词:

对DNase的耐药性进行研究.皮肤腐烂是一种腐烂.牙斑生物膜是一种牙斑生物膜.我们的eDNADNA 细胞外矩阵是细胞外矩阵.左撇子的 DNA 是左撇子的 DNA

更多相关视频

Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

相关概念视频

Adherens Junctions

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Intracellular Movement of Viruses and Bacteria

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...