Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression.

Nature communications·2026
Same author

Novel pESI-encoded autotransporter adhesin PeaP of epidemic <i>Salmonella</i> strains mediates adhesion, atypical biofilm formation, and poultry colonization.

bioRxiv : the preprint server for biology·2026
Same author

Intracellular lipopolysaccharide binds RETREG1/FAM134B to regulate ER remodeling upon bacterial infection.

Autophagy·2026
Same author

Any1 is a phospholipid scramblase involved in endosome biogenesis.

The Journal of cell biology·2025
Same author

Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus.

The European respiratory journal·2024
Same author

Non-professional efferocytosis of Salmonella-infected intestinal epithelial cells in the neonatal host.

The Journal of experimental medicine·2024

相关实验视频

Updated: May 22, 2025

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

8.5K

实验方法可视化在宿主-病原体相互作用期间的效应蛋白转位.

Verena Nadin Fritsch1, Michael Hensel1,2

  • 1Abt. Mikrobiologie, Universität Osnabrück, Osnabrück, Germany.

BioEssays : news and reviews in molecular, cellular and developmental biology
|March 13, 2025
PubMed
概括

细菌病原体将效应蛋白注入宿主细胞,这是疾病的关键一步. 本综述详细介绍了研究效应蛋白转位的工具,有助于理解细菌病原性.

关键词:
这就是T3SS.这就是T4SS.在T6SSSS中使用.效应器效应器是一个有效的效应器.自标签酶的自标签酶.转移转移是指转移的转移.

更多相关视频

Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
10:29

Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

Published on: July 6, 2016

10.6K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.4K

相关实验视频

Last Updated: May 22, 2025

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

8.5K
Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
10:29

Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

Published on: July 6, 2016

10.6K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.4K

科学领域:

  • 微生物学 微生物学
  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 细菌病原体利用复杂的分泌系统将效应蛋白送入宿主细胞.
  • 效应器转移对于操纵宿主细胞和驱动病原发生是必不可少的.
  • 了解效应蛋白的功能需要分析它们的转位动态,定位和目标.

研究的目的:

  • 为研究细菌效应蛋白转位提供生物化学和遗传工具的概述.
  • 讨论挑战和分析效能者转移的各种方法.
  • 突出该领域最近的进展和未来的方向.

主要方法:

  • 审查现有的生物化学和遗传工具.
  • 描述固定细胞中的静态可视化技术.
  • 讨论动态活细胞成像和单分子追踪方法.

主要成果:

  • 有各种方法,从静态到动态活细胞成像,用于研究效应器转位.
  • 最近的进展允许实时,单分子追踪效应蛋白质.
  • 每种方法在分析转移方面都有明显的优势和局限性.

结论:

  • 有效的工具可用于研究细菌效应蛋白转位.
  • 活细胞成像和单分子技术为效应器动态提供了强大的洞察力.
  • 需要进一步应用先进的方法来解决宿主-病原体相互作用中的未解决的问题.