主要的植物性大麻素,三角-9-四大麻 (THC) 和大麻 (CBD),影响人类精子中的CatSper通道的功能
Lydia Wehrli1,2, Hannah Altevogt3, Christoph Brenker3
1Department of Genetic Medicine and Development, University of Geneva, Geneva, Switzerland.
德尔塔-9-四大麻 (THC) 和大麻 (CBD) 通过影响CatSper通道影响人类精子功能. 这些大麻素抑制了关键的精子激活通路,THC显示出更大的功效和避孕药开发的潜力.
科学领域:
- 生殖生物学和毒理学.
- 对于大麻素的研究.
- 精子生理学 精子生理学
背景情况:
- 大麻sativa是一种广泛使用的娱乐药物.
- 关于植物性大麻素和精液参数的先前研究存在,但THC和CBD对人类精子功能的直接影响尚不清楚.
研究的目的:
- 研究delta-9-tetrahydrocannabinol (THC) 和cannabidiol (CBD) 对人类精子功能的直接影响.
- 确定THC和CBD对精子特异性通道,CatSper及其激活通路的影响.
主要方法:
- 研究了通过CatSper在人类精子中的Ca2+流入,使用动力Ca2+度和补丁记录.
- 评估了THC和CBD对孕激素 (P4),前列腺素E1 (PGE1) 和pH诱导的CatSper激活的影响.
- 评估了对THC和CBD的反应中的精子运动性,透能力和青体反应 (AR).
主要成果:
- THC和CBD都调节了CatSper活性,THC和CBD抑制了P4和PGE1诱导的Ca2+涌入.
- 与CBD相比,THC在抑制CatSper激活方面表现出更高的功效,并且也影响了精子的过度激活和透.
- THC和CBD作为真正的CatSper抑制剂,THC的功效超过了大多数已知的抑制剂.
结论:
- THC和CBD与CatSper通道相互作用,可能会干扰人类受精.
- THC对精子功能具有显著的抑制作用,可以作为开发新型非激素避孕药的基础.
- 需要进一步的体内研究来确定大麻消费对生育能力的影响.
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