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探索肠道微生物群和肺动脉高血压之间的因果关系:从孟德尔随机化中的见解

Lihuang Su¹, Xinghong Wang¹, Ya Lin¹

¹Division of Pulmonary Medicine, Wenzhou Key Laboratory of Interdisciplinary and Translational Medicine The First Affiliated Hospital of Wenzhou Medical University Wenzhou Zhejiang China.

Journal of the American Heart Association

|March 13, 2025

概括

肠道微生物组成通过改变肺基因表达来影响肺动脉高血压 (PAH). 特定的肠道细菌和代谢物与PAH的发展有关,提供潜在的诊断和治疗点.

科学领域:

微生物组研究的研究.
肺血管疾病是肺血管疾病.
遗传学遗传学是一个

背景情况:

肠肺轴将肠道微生物群与肺动脉高血压 (PAH) 连接起来.
连接肠道微生物群与PAH病原体的机制尚未完全理解.

研究的目的:

使用孟德尔随机化研究肠道微生物群和PAH之间的因果关系.
确定PAH中涉及的关键微生物群和代谢物.
探索PAH病变发生过程中相关基因的调节作用.

主要方法:

两个样本的孟德尔随机化分析 (反差加权,MR-Egger,加权中位数,加权模式).
生物信息学用于识别肠道微生物群中的PAH调节基因.
定量逆转录聚合酶链反应 (qRT-PCR) 用于验证小鼠模型中的基因表达.

主要成果:

11种肠道微生物群表现出与PAH的关联,其中一些表现出双向因果关系.
特定的肠道细菌,包括Clostridiales和Bifidobacteriales,与PAH有关.
相关的微生物群调节24种代谢物,影响PAH.
在PAH小鼠的肺组织中观察到的特定基因 (例如1,4,5-三酸盐受体2型) 的下调.

结论:

肠道微生物群的组成和代谢物通过调节肺部基因表达来促进PAH.
这些发现支持开发基于肠道微生物群的诊断工具和针对PAH的向治疗方法.

关键词:

门德尔的随机化分析分析.基因调节基因调节基因调节我们的肠道微生物群.代谢产物的代谢产物肺高血压是一种肺高血压.

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