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对循环细胞自由线粒体和核DNA度的方法影响,以应对慢性压力

Carina Daubermann¹, Benedict Herhaus², Elmo W I Neuberger¹

¹Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University Mainz, Albert-Schweitzer Strasse 22, 55128, Mainz, Germany.

Molecular biology reports

|March 13, 2025

概括

无细胞循环线粒体DNA (ccf-mtDNA) 可以在血中定量,可能表明慢性压力. 然而,健康个体的水平接近量化的极限,需要进一步细化分析.

科学领域:

生物化学生化学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

线粒体是参与细胞应激反应的重要器官.
慢性压力可能会损害线粒体,释放无细胞循环线粒体DNA (ccf-mtDNA) 进入血液.
ccf-mtDNA是细胞损伤和压力的潜在生物标志物.

研究的目的:

研究ccf-mtDNA和无细胞循环核DNA (cf-nDNA) 作为健康个体慢性压力的生物标志物的实用性.
开发和验证可靠的定量聚合酶链反应 (qPCR) 试验,用于ccf-mtDNA测量.

主要方法:

根据国际指导方针 (FDA,ISO,CLSI) 在人体血中测量ccf-mtDNA的qPCR试验进行了开发和验证.
通过产生的样本分析和板间可变性测试来评估测试可靠性.
评估了离心力对ccf-mtDNA和cf-nDNA度的影响.

主要成果:

开发的qPCR试验可靠地量化未净化血中的ccf-mtDNA.
离心力显著影响ccf-mtDNA水平,但不影响cf-nDNA.
在ccf-mtDNA水平和慢性压力之间观察到显著的负相关性.

结论:

ccf-mtDNA是血中的可量化的生物标志物,反映了慢性压力.
测试灵敏度有限,因为健康受试者的ccf-mtDNA水平接近量化极限 (LOQ).
为了更广泛的临床适用性,需要进一步的测试优化.

关键词:

测试开发的发展.生物标志物生物标志物慢性压力慢性压力循环细胞自由的线粒体DNA 循环细胞的自由核DNA是循环细胞的 DNA.

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