高通量固体相提取用于有针对性和非有针对性的暴露物
Yunyun Gu1,2, Max Lennart Feuerstein1,3, Benedikt Warth1,2,3
1Faculty of Chemistry, Department of Food Chemistry and Toxicology, University of Vienna, 1090 Vienna, Austria.
Analytical chemistry
|March 13, 2025
概括
一种新的固相提取 (SPE) 方法改善了尿液和血中的化学暴露组分析. 这种强大,可扩展的协议提高了环境污染物检测的吞吐量,有助于暴露全组关联研究.
科学领域:
- 环境化学环境化学
- 分析化学是一种分析化学.
- 毒理学 毒理学 毒理学
背景情况:
- 化学暴露体的特征对于理解环境健康影响至关重要.
- 目前的暴露学样本预处理方法在分析剂覆盖率,稳定性和吞吐量方面存在瓶.
- 先进的仪器仪表,如液态色谱-并联质谱 (LC-MS/MS) 需要优化样品的准备.
研究的目的:
- 为人类尿液和血开发和优化一个强大的和可扩展的固相提取 (SPE) 协议.
- 评估各种环境和食品相关污染物的提取效率和信号抑制/增强.
- 为了提高暴露组分析的吞吐量,进行大规模研究,如暴露组范围的关联研究 (ExWAS).
主要方法:
- 开发了人类尿液和血的固相提取 (SPE) 协议.
- 为94种不同的环境和食品相关污染物优化了SPE.
- 使用目标LC-MS/MS来确定提取回收 (RE) 和信号抑制/增强 (SSE).
- 将该方法转移到96井板格式,用于高通量分析.
- 使用NIST标准参考材料,将SPE方法与蛋白质沉进行比较.
主要成果:
- 在70%以上的分析物中实现了可接受的提取回收 (60-140%).
- 达到可接受的信号抑制/增强 (60-140%) 86%的尿液分析物和90%的血分析物.
- 96井板格式显著增加了吞吐量,估计比1000个样本的蛋白质沉速度快10倍.
- 该SPE协议在非目标分析 (NTA) 和嫌疑人查方面显示出有希望的性能.
结论:
- 开发的SPE工作流是强大的,可扩展的,并显著提高了针对目标曝光的吞吐量.
- 这种方法为非目标分析 (NTA) 和曝光学中可疑查提供了可行的选择.
- 优化的协议适用于各种研究领域,包括药理学,食品安全和系统毒理学.
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