脊柱TRPC3促进神经病痛,并协调脂酶C诱导的机械过敏
Shota Tobori1, Kosei Tamada1, Nagi Uemura1
1Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
概括
脊柱背角神经元中的暂时受体潜在正规3 (TRPC3) 通道对于神经损伤后发生神经病痛至关重要. TRPC3为这种情况提供了潜在的治疗点.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 分子生物学分子生物学
背景情况:
- 神经病痛,通常是由于外围神经损伤造成的,由于不清楚的潜在机制,它带来了重大挑战.
- 暂时受体潜在的正规3 (TRPC3) 通道,TRP通道家族的一部分,由Gq-脂酶C (PLC) 途径激活,存在于体感系统中.
研究的目的:
- 研究TRPC3在经外围神经损伤后神经病痛的发展中的作用.
- 为了确定脊柱背角神经元中TRPC3是否有助于疼痛过敏.
主要方法:
- 在野生型和TRPC3-Knockout (TRPC3-KO) 小鼠中的部分坐骨神经绑定 (pSNL) 模型.
- 在现场杂交以评估TRPC3表达在脊柱背角.
- 微RNA (miRNA) 介导的TRPC3在脊柱背角神经元中的下调.
- 机械和热过敏度的评估.
主要成果:
- pSNL在野生型小鼠中诱导了机械和热过敏,在TRPC3-KO小鼠中显著降低.
- TRPC3主要表达在脊柱背部角内的神经元中.
- 在脊柱背角神经元中TRPC3的下调减弱了PSNL诱导的过敏.
- 脊柱TRPC3的激活诱导了急性机械过敏性,其遗传除减少了NK1R或PLC激活诱导的过敏性.
结论:
- 脊柱背角神经元中的TRPC3通道在促进神经病痛的发展和维持方面发挥着至关重要的作用.
- TRPC3代表了一种有前途的治疗点,用于管理由外围神经损伤引起的神经病痛.
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