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治疗剂量的普雷尼索隆对小鼠卵细胞能力没有不利影响

Shruthivishali Muthukumar¹, Vijeta Shetty¹, Akshatha Daddangadi¹

¹Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal-576 104, India.

JBRA assisted reproduction

|March 13, 2025

概括

短期暴露于PRDL (一种药物) 并没有损害小鼠卵细胞的发育,尽管高剂量引起了子问题. 需要进一步研究PRDL的长期影响.

科学领域:

生殖生物学生殖生物学
发育毒理学发展毒理学
药理学药理学是指药理学的学科.

背景情况:

卵细胞发育能力对于成功繁殖至关重要.
了解治疗剂对卵细胞质量的影响对于患者安全至关重要.
PRDL是一种用于可能影响生殖结果的治疗中的药物.

研究的目的:

研究PRDL对小鼠卵细胞发育能力的影响.
评估不同剂量的PRDL对卵细胞质量和随后胚胎发育的影响.
评估PRDL对卵细胞成熟,线形成和激活的直接影响.

主要方法:

在超排卵期间,瑞士白色小鼠接受了不同剂量的PRDL.
从接受治疗的小鼠中评估了卵细胞质量和胚胎细胞发育.
在体外成熟过程中,卵子被暴露在PRDL中,以评估线和激活潜力.

主要成果:

与对照组相比,PRDL的使用增加了芽细胞的总细胞数.
布拉斯托囊中亡的发生率在PRDL暴露和对照组之间仍然可比.
直接暴露于10μM的PRDL显著增加了卵细胞轴异常 (p<0.05).

结论:

短期的治疗性PRDL暴露似乎对体外卵细胞发育潜力是安全的.
虽然PRDL在这种小鼠模型中没有显示任何有害影响,但建议进一步研究长期暴露.
由于其在临床环境中广泛使用,长期PRDL暴露研究是有必要的.

关键词:

辅助生殖技术是一种辅助生殖技术.葡萄糖皮质类药物是什么卵子细胞卵子细胞普雷迪尼索隆 (prednisolone) 是一种药物.植入前胚胎的胚胎发育

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