circEgg通过调节H3K9me3和H3K9ac之间的过渡来抑制BmCPV感染
Qunnan Qiu1, Xinyu Tong1, Min Zhu2
1School of Life Sciences, Soochow University, Suzhou 21523, China.
Pesticide biochemistry and physiology
|March 13, 2025
概括
循环RNAcircEgg通过调节组织蛋白修饰物来抑制Bombyx mori细胞质多病毒 (BmCPV) 感染. 这项研究显示,circEgg增强了H3K9ac并减少了H3K9me3,这对抗病毒防御至关重要.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 循环RNAs (circRNAs) 是生物过程中的新兴调节者.
- 博姆比克斯·莫里细胞质多重体病毒 (BmCPV) 对丝虫的生长构成威胁.
- circEgg在BmCPV感染反应中的作用以前是未知的.
研究的目的:
- 为了阐明circEgg和BmCPV感染之间的功能关系.
- 为了研究circEgg在抗病毒防御中的作用背后的分子机制.
- 探索circEgg对宿主基因表达和病毒感染期间表观遗传修饰的影响.
主要方法:
- 定量实时PCR测量基因和circRNA表达.
- 暂时表达试验评估circEgg对BmCPV.的抑制作用.
- 西部涂抹以分析基因素修饰 (H3K9me3和H3K9ac).
- 生物信息分析以确定涉及circEgg的监管网络.
主要成果:
- 在BmCPV感染后,CircEgg表达显著增加,而其宿主基因BmEgg则下降.
- 证实CircEgg可以抑制BmCPV感染.
- BmCPV感染改变了基因素甲基化 (H3K9me3) 和乙化 (H3K9ac) 的水平.
- CircEgg与H3K9ac正相关,与H3K9me3负相关,这表明它们在平衡中的作用.
- 机械研究表明,circEgg通过miR-3391-5p-Rpd3通路调节H3K9ac,通过circEgg-P122.3调节H3K9me3.
结论:
- CircEgg在抑制丝虫BmCPV感染方面发挥着至关重要的作用.
- CircEgg通过调节H3K9me3和H3K9ac.之间的表观遗传平衡来调节病毒防御.
- 这种circEgg-bmo-miR-3391-5p-Rpd3和circEgg-P122通路是circEgg抗病毒活性的关键机制.
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