阿米苏尔布朗诱导线粒体功能障碍,导致人类热囊细胞和子宫内膜细胞的亡和细胞循环停止
Miji Kim1, Wonhyoung Park2, Whasun Lim3
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Pesticide biochemistry and physiology
|March 13, 2025
概括
阿米苏尔布朗杀菌剂暴露会导致细胞损伤,影响植入. 这项研究揭示了通过破坏人类子宫内膜细胞和热囊细胞对怀孕的潜在风险,强调了需要进一步调查的必要性.
科学领域:
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
- 环境科学 环境科学
背景情况:
- 阿米苏是一种用于增强农业生产的三醇杀菌剂.
- 有关其长时间的环境持久性 (DT50) 和对非标生物的毒性存在担忧.
- 艾米苏对人类怀孕过程的特定影响仍然在很大程度上是未知的.
研究的目的:
- 研究阿米苏对人类热囊细胞 (HTR-8/SVneo) 和子宫内膜细胞 (T HESCs) 的细胞毒性作用.
- 阐明在植入关键细胞中阿米苏诱导的毒性的潜在机制.
主要方法:
- 对HTR-8/SVneo和T HESC细胞系暴露于阿米苏.
- 评估线粒体功能,细胞内水平和细胞周期进展.
- 对活性氧物种 (ROS) 生产和PI3K/AKT信号通路的分析.
- 在3D悬浮滴培养模型中评估球形形成.
主要成果:
- 在两种细胞类型中,amisulbrom诱导了线粒体功能障碍和细胞内过载.
- 暴露导致G2/M阶段的细胞周期停止,增加了细胞亡,并降低了细胞活力.
- 观察到ROS水平升高和PI3K/AKT信号蛋白的脱化.
- 氨硫抑制了球状体的形成,这表明植入潜力受损.
结论:
- 阿米苏尔布朗对人体热囊细胞和子宫内膜细胞表现出细胞毒性作用,可能破坏植入过程.
- 机制包括线粒体功能障碍,过载,ROS生成和PI3K/AKT通路干扰.
- 这项研究引起了人们对阿米苏的生殖毒性的担忧,需要进一步的体内研究.
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