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Updated: May 22, 2025

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使用悬浮中断标记在现场和测序 (sBLISS) 精确地绘制生理DSB

Osama Hidmi1, Sara Oster1, Diala Shatleh1

  • 1The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research-IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.

Methods in molecular biology (Clifton, N.J.)
|March 14, 2025
PubMed
概括

生理学DNA双链断裂 (DSB) 对正常细胞功能至关重要,但如果错误修复,可能导致基因组不稳定. 这项研究详细介绍了sBLISS方法,用于精确地绘制这些重要的DNA断裂.

关键词:
打破了一些.在DSBs中使用DSBs.内生DNA损伤是内生DNA损伤.这是下一代测序.衰老是一种衰老.这就是为什么SBLISSISS.

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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