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Updated: May 22, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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在Orbitrap Astral质谱仪上使用狭窄窗口数据独立获取的深度等离子体N-糖蛋白质组分析

Shelley Jager1, Martin Zeller2, Anna Pashkova2

  • 1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands.

Nature communications
|March 14, 2025
PubMed
概括

新的Orbitrap Astral质谱仪能够使用新的狭窄窗口数据独立获取 (nGlycoDIA) 工作流程快速深入分析人体血N-糖蛋白组. 这种高通量方法可以识别成千上万的糖和糖蛋白,推进临床糖蛋白组学.

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相关概念视频

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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