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在植物发育和再生过程中通过全合一的CRE/LOX系统追踪干细胞细胞系

Shengnan Wang¹, Yu Li^2,3, Zijuan Li²

¹School of Life Sciences, East China Normal University, Shanghai, 200241, China.

|March 14, 2025

概括

一个新的CRE/LOX系统有效地追踪植物干细胞系在根部和芽部的角干细胞系统. 这个工具揭示了静止中心细胞是如何补充干细胞的,以及祖先细胞是如何形成新的芽细胞系统的.

科学领域:

植物发育生物学植物发育生物学
分子遗传学分子遗传学
细胞生物学细胞生物学

背景情况:

根垂干 (RAM) 和芽垂干 (SAM) 对于植物生长至关重要,它们含有形成新器官的干细胞.
了解干细胞谱系的追踪对于理解干细胞系统的形成和器官生成至关重要.

研究的目的:

引入一个全集的CRE/LOX系统,以有效地追踪Arabidopsis thaliana的细胞系.
在发育和再生过程中研究RAM和SAM的干细胞动态.

主要方法:

开发一个单等离子体全合一的CRE/LOX系统.
使用WOX5促销器驱动的CRE/LOX-GUS在RAM中进行血统追踪.
使用CRE/LOX-RUBY进行实时体内血统追踪.

主要成果:

WOX5_pro-CRE/LOX-GUS系统证明,静止中心 (QC) 细胞分裂可能会补充RAM中的干细胞.
该系统还表明,射击原生细胞合作在新生再生过程中启动SAM.
通过CRE/LOX-RUBY系统,可以实时观察体内细胞系.

结论:

全合一的CRE/LOX系统为植物细胞谱系追踪提供了一个有效的工具.
这项研究阐明了参与Arabidopsis. meristem发育和再生的细胞系.
这些发现提供了对干细胞补充和de novomeristem形成的见解.

关键词:

阿拉比多普西斯塔利亚纳.在WOX11上使用.在WOX5上,WOX5是什么?在CRE/LOX中使用.细胞谱系细胞谱系优质系统优质系统植物再生植物再生红宝石 (Ruby) 是一种红宝石.

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