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通过核磁共振旋转放松与Zwitterionic纳米粒子相互作用时球状蛋白的动力学

Xinyao Xiang¹, Lei Bruschweiler-Li¹, Joseph B Schlenoff²

¹Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, United States.

Journal of the American Chemical Society

|March 14, 2025

概括

用zwitterionic分子覆盖纳米粒子改变了蛋白质的相互作用. 这使得蛋白质可以在纳米粒子表面移动,类似于脂质体相互作用,使得量身定制的纳米粒子功能成为可能.

科学领域:

纳米粒子与生物分子的相互作用
蛋白质动力学和生物物理学
表面化学和材料科学

背景情况:

描述纳米粒子与蛋白质相互作用对于化学和生物学新兴应用至关重要.
核磁共振 (NMR) 旋转放松对纳米粒子相互作用时的蛋白质移动性和结合动力学具有敏感性.
蛋白质与纳米粒子的相互作用不同于脂质组纳米粒子,尽管两者都是静电驱动的.

研究的目的:

调查如何涂层纳米粒子与zwitterionic分子影响蛋白质相互作用.
将涂层纳米颗粒上的蛋白质动态与脂质体上的蛋白质动态进行比较.
为量身定制的应用了解蛋白质-纳米粒子相互作用的机制.

主要方法:

使用的核磁共振 (NMR) ¹⁵N横向R₂放松测量
研究了乌比基和B-Raf与修饰的纳米粒子的Ras结合域的相互作用.
涂层纳米颗粒与未经修改的和脂质组纳米颗粒的蛋白质动力学比较.

主要成果:

涂上硫乙烯西洛二氧化分子改变了蛋白质相互作用,使其与脂质体相互作用相似.
蛋白质 (ubiquitin,B-Raf) 在涂层的纳米粒子表面表现出轴向重定向运动.
互动主要是带有正电荷的表面区域,表明全球动态机制.

结论:

在纳米颗粒上的Zwitterionic涂层诱导与脂质体相似的蛋白质动力学.
蛋白质在具有密集电荷的软表面的纳米粒子上经历全球动态.
定制纳米粒子涂层可以控制特定的体外和体内功能中的蛋白质-纳米粒子相互作用.

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