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在光照下培养的竹悬浮细胞中发现咖啡基酸

Naoki Ube¹, Yasuo Kato¹, Taiji Nomura¹

¹Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.

Plant biotechnology (Tokyo, Japan)

|March 14, 2025

概括

竹子细胞可以被设计成产生有价值的化合物,如酸. 这项研究优化了竹 (Phyllostachys nigra; Pn) 细胞培养的条件,实现了这些重要的自然产品的高产量.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
自然产品生物合成自然产品生物合成

背景情况:

合理的代谢流切换重定向了植物细胞培养物中外源性化合物生产的固有途径.
竹子 (Phyllostachys nigra; Pn) 细胞已经显示出产生来自费鲁和p-coumaroyl-CoAs的烯化合物的潜力.

研究的目的:

评估Pn细胞在不同的培养条件下产生外源代谢物的代谢潜力.
识别和优化Pn悬浮细胞中光诱导化合物的生产条件.

主要方法:

在光照条件下培养Pn悬浮细胞.
积累的化合物的隔离和识别.
优化培养条件以最大限度地提高生产量.

主要成果:

PN悬浮细胞积累了两个主要的光诱导化合物:3-O-咖啡酸 (新酸) 和5-O-咖啡酸 (酸).
优化的培养条件导致3-O-caffeoylquinic 酸的产量标位为121 mg/L,5-O-caffeoylquinic 酸的产量标位为77.9 mg/L.
这些结果凸显了PN细胞在生物生产中的潜力.

结论:

PN细胞是外源代谢物的生物生产的合适宿主,特别是那些来自咖啡-CoA.
合理的代谢流切换策略可以有效地应用于PN细胞,用于生产有价值的化合物.
进一步优化可以提高Pn细胞培养中的特定代谢物的产生.

关键词:

菲洛斯塔克斯黑色的黑色动物竹子细胞是一种细胞.原酸是原酸中的一种.基辛纳基基因酸基辛纳基因酸光诱导的二次代谢物.

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