使用拉曼显微光谱检测辐射敏感子群ex-vivo
Aidan D Meade1, Adrian Maguire1,2, Jane Bryant2
1School of Physics, Clinical and Optometric Sciences, Technological University Dublin, Dublin, Ireland.
Frontiers in oncology
|March 14, 2025
概括
拉曼光谱对检测患者的放射敏感性显示出希望. 这种技术可以识别敏感的细胞群体,可能有助于使用液体活检个性化癌症治疗策略.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 癌症研究 癌症研究
- 放射生物学的放射生物学
背景情况:
- 了解辐射敏感性对于有效的癌症治疗至关重要.
- 目前用于检测放射性敏感性的方法有限.
- 拉曼光谱已经成为生物分析的一个有前途的工具.
研究的目的:
- 研究拉曼光谱作为检测和分类放射敏感性的方法.
- 评估机器学习模型在区分放射敏感细胞系中的性能.
- 探索拉曼光谱在液体活检应用中的潜力.
主要方法:
- 拉曼光谱法被用来分析从患者的淋巴状细胞细胞系的分析 无氧电脉切开症,非霍奇金斯淋巴瘤,特纳综合征,健康对照和前列腺癌患者.
- 细胞样本暴露于不同剂量的X射线辐射 (0 Gy,50 mGy,500 mGy).
- 使用线性 (PCA) 和非线性 (UMAP) 缩小维度的支向量机模型被开发用于分类光谱数据.
主要成果:
- 拉曼光谱与机器学习相结合,成功地区分了辐射敏感和非辐射敏感的细胞群.
- 与线性方法 (PCA) 相比,非线性维度减少 (UMAP) 改善了歧视.
- 在非辐射样本 (0 Gy) 上训练的模型在分类细胞类型方面取得了最高的性能 (F1 = 0.92 ± 0.06).
结论:
- 拉曼光谱具有作为评估内在辐射敏感性的非侵入性工具的潜力.
- 这项技术可能能够识别个性化医疗的辐射敏感亚群.
- 进一步的研究可以验证拉曼光谱在液体活检中临床使用.
相关概念视频
01:20Raman Spectroscopy: Overview
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
01:26Raman Spectroscopy Instrumentation: Overview
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...