成功的玻璃化人类骨质状骨和完整的股骨状骨
Mohammadhamed Shahsavari1, Shannon Clark2, Janet A W Elliott2,3
1Department of Surgery, University of Alberta, Edmonton, AB, Canada.
Cartilage
|March 14, 2025
概括
玻璃化协议有效地保护人类关节软骨 (AC) 进行移植. 两种测试方法都保持了高的冠状细胞活力和组织质量,为关节缺陷治疗推进了AC冷保存.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 关节软骨 (AC) 移植对于治疗大关节缺陷至关重要.
- 供体AC的有限可用性限制了其临床应用.
- 通过玻璃化进行冷保存提供了一个有前途的解决方案,以增加AC移植的可用性.
研究的目的:
- 为了比较两个冷保存方案对人类关节软骨的疗效.
- 评估玻璃化对状细胞活力和组织质量的影响.
- 评估玻璃化AC在未来临床应用中的潜力.
主要方法:
- 从3个捐赠者获得的人类骨质突 (10毫米直径) 经过使用第8协议或第2BWF协议进行玻璃化.
- 在升温后评估了慢性细胞活力,并将其正常化为新鲜对照.
- 协议2BWF进一步在完整的人类大腿上进行了测试,随后对代谢活性,组织学和矩阵生产力进行了分析.
主要成果:
- 协议8和协议2BWF在玻璃化和重新加热后都实现了高状细胞活力 (新鲜对照的80%左右).
- 使用2BWF协议进行完整的股骨状体的玻璃化和重新加热,导致约82%的冠状细胞活力.
- 玻璃化AC样本显示了与新鲜对照对比的代谢活性,组织学和矩阵生产力.
结论:
- 议定书8和议定书2BWF是通过玻璃化对人类关节软骨进行冷保存的有效方法.
- 这两种协议都保持了骨质突和完好无损的股骨骨的玻璃化人体AC的质量.
- 这些发现支持AC玻璃化的进步,以提高在治疗关节缺陷中的移植可用性.
相关概念视频
01:55Bone Structure
Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
01:27Growth of Cartilage and Bone Tissue
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
01:23Bone as Supporting Connective Tissue
Bone tissue forms the internal skeleton of vertebrate animals, providing structure to the body.
Bone Matrix
Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts— that give the...
Bone Matrix
Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts— that give the...
01:18Classification of Bones
The bones of the human skeletal system are of varied shapes, sizes, and functions. They can be classified based on their shape and function into four major classes: long bones, short bones, flat bones, and irregular bones. Some classifications include a fifth type, the sesamoid bones, as a separate class, whereas others categorize them under short bones.
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The long...
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The long...
01:27Compact Bone
Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
01:09Spongy Bone
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...