相关实验视频

Updated: May 22, 2025

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

在皮动物中,皮细胞的死亡是通过裂变调整的,但不是MLKL的酸化

Kaiyu Chen¹, Sikou Shen¹, Zhimeng Lv¹

¹State Key Laboratory of Agricultural Products Safety, Ningbo University, Ningbo, People's Republic of China.

|March 14, 2025

概括

研究人员在海黄瓜中发现了一种新的细胞死亡途径,涉及MLKL裂变,而不是酸化. 这一发现揭示了无脊椎动物免疫力和细胞死亡进化的新机制.

科学领域:

细胞生物学细胞生物学
免疫学免疫学免疫学
进化生物学进化生物学

背景情况:

哺乳动物中的性细胞死亡涉及MLKL酸化 (死) 或Gasdermin裂变 (烧).
无脊椎动物拥有独特的免疫机制,对它们的临床细胞死亡途径的理解有限.

研究的目的:

在海黄瓜Apostichopus japonicus中识别和表征新型细胞死亡调节剂.
为了阐明由A. japonicus.中Vibrio splendidus感染引起的细胞死亡的机制.

主要方法:

基因组查以确定MLKL同类物.
在对细菌感染的反应中对AJMLKL的功能分析.
生物化学试验以确定AJMLKL裂变和膜相互作用.
炎症一些复杂的形成分析.

主要成果:

在A. japonicus中发现了一种新的MLKL同类物 (AjMLKL),缺乏Gasdermin,但具有形裂解图案.
通过AJCASP-1的分裂,AjMLKL通过RIPK3-介导的酸化独立地调解解性细胞死亡.
分裂的AJMLKL直接破坏了膜的完整性,形成毛孔并杀死细菌.
炎症性AJCASP-1激活取决于一个涉及AJNLRC4的炎症类复合体.

结论:

这项研究提供了MLKL在皮动物中依赖于裂变的性细胞死亡的第一个证据.
这些发现揭示了无脊椎动物中MLKL功能独特的进化路径.
这项工作提供了对不同物种天生的免疫和细胞死亡调节的多样化机制的见解.

更多相关视频

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy

Published on: January 9, 2019

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

相关概念视频

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Overview of Cell Death

Overview of Cell Death

Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...