Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

12.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
12.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluation of a polarization-sensitive, dual-wavelength wearable photoplethysmography sensor across a range of skin tones.

Biophotonics discovery·2026
Same author

Smartphone tristimulus colorimetry for skin-tone analysis at common pulse oximetry anatomical sites.

Biophotonics discovery·2026
Same author

EVALUATION OF THE INSECT GROWTH REGULATOR SUMILARV 0.5G AGAINST ANOPHELES COLUZZII AND AN. ARABIENSIS LARVAE UNDER LABORATORY AND SEMIFIELD CONDITIONS.

Journal of the American Mosquito Control Association·2026
Same author

Emerging Technologies for Multiphoton Writing and Reading of Polymeric Architectures for Biomedical Applications.

Annual review of biomedical engineering·2025
Same author

Induced pluripotent stem cell-derived cardiomyocyte in vitro models: benchmarking progress and ongoing challenges.

Nature methods·2024
Same author

Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.

Journal of biomedical optics·2024

相关实验视频

Updated: May 22, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

9.7K

通过可编程的时空光片获得较大的焦点深度.

Mbaye Diouf, Mitchell Harling, Kimani C Toussaint

    Optics letters
    |March 14, 2025
    PubMed
    概括

    时空光片抵抗衍射,在毫米范围内保持一致的宽度. 这为光板显微镜等应用提供了扩展的焦点深度.

    科学领域:

    • 光学物理学 光学物理学
    • 生物医学光学 生物医学光学

    背景情况:

    • 时空波包表现出无衍射的传播,吸引了光学物理学的重大兴趣.
    • 这种无衍射的特性具有生物医学光学应用的潜力.

    研究的目的:

    • 通过实验和数量证明时空光片的抗衍射性能.
    • 为了比较时空光片的传播特征与艾里,贝塞尔和高斯光片.

    主要方法:

    • 通过调整光的光谱倾斜角度来合成10μm厚的时空光片.
    • 通过实验和数值测量时空,空气,贝塞尔和高斯光板在2毫米传播距离上的宽度.

    主要成果:

    • 时空光板在没有聚焦镜头的情况下在2毫米的自由空间传播中保持了10微米的宽度.
    • 具有相似初始厚度的空气,贝塞尔和高斯光片显著分离,在相同的距离上变得4.5×到10×更宽.

    结论:

    • 与传统的光板类型相比,时空光板显示出更高的衍射电阻.
    • 这些发现表明时空光片是实现光片显微镜扩展焦点深度的有希望的工具.

    更多相关视频

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.7K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.4K

    相关实验视频

    Last Updated: May 22, 2025

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.7K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    9.7K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.4K