病理学和细菌学调查Pasteurella multocida诱导的牛犊疹炎
Kaori Namba1, Aya Terao2, Yuichi Ueno3
1Okayama Prefectural Okayama Livestock Hygiene Service Center, Okayama, Okayama, Japan.
Veterinary microbiology
|March 14, 2025
概括
通常是呼吸道病原体的Pasteurella multocida被确定为日本黑牛小牛牛皮膜炎的原因. 这项研究证实了它通过上升感染在生殖问题上的作用.
科学领域:
- 兽医病理学 兽医病理学
- 牛的繁殖 牛的繁殖
- 细菌病原体的产生
背景情况:
- 帕斯托雷拉多 (P. multocida) 是已知的牛呼吸系统疾病的原因之一.
- 它在牛生殖障碍,特别是脊髓炎中的作用尚未得到充分研究.
- 这项研究调查了P. multocida作为导致小牛膜炎的病因.
研究的目的:
- 证实P. multocida是牛群中流行性炎症的原因.
- 为了阐明感染路径和P. multocida诱导的流行病的病理特征.
- 为了确定从受影响的小牛中分离出来的P. multocida的遗传特征.
主要方法:
- 在日本多个农场的小牛中确诊了疫苗感染病例.
- 对受影响的 epididymal 组织进行了 histopathological 和 immunohistochemical 的分析.
- 细菌分离,多位置序列类型化 (MLST) 和脉冲场凝电泳 (PFGE) 的P. multocida分离物.
主要成果:
- 在日本黑色小牛中发现了5例P. multocida诱导的流行病炎.
- 组织病理学揭示了带有上升感染的证据的皮格兰瘤性病变.
- 免疫组织化学检查证实了巨细胞和中性粒细胞中的P. multocida血清型A.
- MLST将分离物分为ST79和ST13;PFGE显示出不同的模式,表明不同的起源.
结论:
- 这是第一个通过免疫组织化学方法证实P. multocida参与牛脊髓炎的报告.
- 从口腔或鼻腔上升的感染是最可能的途径.
- 通常在牛的上呼吸道中发现的P. multocida菌株,可以引起脊髓炎,特别是在日本黑牛中.
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